Membrane bilayer balance and erythrocyte shape: a quantitative assessment
- 4 June 1985
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 24 (12), 2849-2857
- https://doi.org/10.1021/bi00333a006
Abstract
When human erythrocytes are incubated with certain phospholipids, the cells become spiculate echinocytes, resembling red cells subjected to metabolic starvation or Ca2+ loading. The present study examines the mode of binding of saturated phosphatidylcholines and egg lysophosphatidylcholine to erythrocytes and the quantitative relationship between phospholipid incorporation and red cell shape. The phospholipids studied become intercalated into erythrocyte membranes, not simply adsorbed to the cell surface. Spin-labeling and radiolabeling data show that the incorporation of (4 .+-. 1) .times. 106 molecules of exogenous phosphatidylcholine/cell converts discocytes to stage 3 echinocytes with .apprx. 35 conical spicules. This amount of lipid incorporation is estimated to expand the red cell membrane outer monolayer by 1.7% .+-. 0.6%. Calculations of the inner and outer monolayer surface areas of model discocytes and stage 3 echinocytes yield an estimated difference of 0.7% .+-. 0.2%.This publication has 23 references indexed in Scilit:
- Solubility of amphipathic molecules in biological membranes and lipid bilayers and its implications for membrane structureBiochemistry, 1981
- Effect of dimyristoyl phosphatidylcholine on intact erythrocytes. Release of spectrin-free vesicles without ATP depletionBiochimica et Biophysica Acta (BBA) - Biomembranes, 1981
- Distribution of shape-changing compounds across the red cell membraneBiochemistry, 1980
- Mechanism of the spontaneous transfer of phospholipids between bilayersBiochemistry, 1980
- Shape changes of human erythrocytes induced by various amphipathic drugs acting on the membrane of the intact cellsBiochemical Pharmacology, 1979
- Relations between membrane monolayers in some red cell shape transformationsJournal of Theoretical Biology, 1978
- A calcium-activated polyphosphoinositide phosphodiesterase in the plasma membrane of human and rabbit erythrocytesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1978
- Selective extraction of membrane-bound proteins by phospholipid vesicles.Journal of Biological Chemistry, 1977
- Production of 1,2-diacylglycerol and phosphatidate in human erythrocytes treated with calcium ions and ionophore A23187Biochemical Journal, 1976
- Red Cell ShapePublished by Springer Nature ,1973