Antigenic relationships among homologous structural polypeptides of porcine, feline, and canine coronaviruses
- 1 September 1982
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 37 (3), 1148-1155
- https://doi.org/10.1128/iai.37.3.1148-1155.1982
Abstract
Transmissible gastroenteritis virus of swine (TGEV), feline infectious peritonitis virus (FIPV), and canine coronavirus were studied with respect to their serological cross-reactivity in homologous and heterologous virus neutralization, immune precipitation of radiolabeled TGEV, electroblotting, and enzyme-linked immunosorbent assay using individual virion polypeptides prepared by polyacrylamide gel electrophoresis. TGEV was neutralized by feline anti-FIPV serum, and the reaction was potentiated by complement; heterologous neutralization involved antibody reacting with the peplomer protein (P), the envelope protein (E), and cellular (glycolipid) components incorporated into the TGEV membrane. Electrophoretic analysis of immune precipitates containing [35S]methionine-labeled disrupted TGEV and feline anti-FIPV antibody confirmed the reaction with the P and E polypeptides and showed the nucleocapsid protein (N) in addition. Electroblotting, followed by incubation with antibody, 125I-labeled protein A, and fluorography, disclosed cross-reactions between the three viruses at the N and E levels and revealed differences in the apparent molecular weights of the latter. Enzyme immunoassays performed with standard amounts of immobilized P, N, and E polypeptides of the three viruses showed recognition of the antigens by homologous and heterologous antibody to comparable degrees. These results indicate a close antigenic relationship between TGEV, FIPV, and canine coronavirus due to common determinants on the three major virion proteins. The taxonomic implications of these findings are discussed.This publication has 20 references indexed in Scilit:
- Antigenic and Biological Relationships between Human Coronavirus OC43 and Neonatal Calf Diarrhoea CoronavirusJournal of General Virology, 1981
- The Polypeptide Structure of Canine Coronavirus and its Relationship to Porcine Transmissible Gastroenteritis VirusJournal of General Virology, 1981
- The virology and pathogenesis of feline infectious peritonitisArchiv für die gesamte Virusforschung, 1979
- The Neutralization of Transmissible Gastroenteritis Virus by Normal Heterotypic SerumJournal of General Virology, 1979
- Antigenic relationship of the feline infectious peritonitis virus to coronaviruses of other speciesArchiv für die gesamte Virusforschung, 1978
- Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized filmFEBS Letters, 1977
- Detection of transmissible gastroenteritis virus neutralising antibody in catsArchiv für die gesamte Virusforschung, 1977
- The Polypeptide Structure of Transmissible Gastroenteritis VirusJournal of General Virology, 1975
- CALF DIARRHŒA CORONAVIRUSThe Lancet, 1975
- Quantitative Film Detection of 3H and 14C in Polyacrylamide Gels by FluorographyEuropean Journal of Biochemistry, 1975