Correlated Effluxes of Adenine Nucleotides, Mg2+ and Ca2+ Induced in Rat‐Liver Mitochondria by External Ca2+ and Phosphate
Open Access
- 1 February 1981
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 114 (2), 195-199
- https://doi.org/10.1111/j.1432-1033.1981.tb05136.x
Abstract
The presence of inorganic phosphate and Ca2+ in the external medium induces a closely parallel efflux of both endogenous adenine nucleotides and Mg2+ from rat liver mitochondria. These effluxes are (a) pH-dependent and inhibited by uncouplers, respiration inhibitors and external Mg2+; (b) completely prevented by bongkrekate, but stimulated by atractylate. ATP, ADP or AMP each inhibit the release of Mg2+ promoted by Ca2+ and phosphate: however, in the presence of oligomycin and P1,P5-di(adenosine-5′)-pentaphosphate (an inhibitor of adenylate kinase) only ADP is effective. Also the release of accumulated Ca2+ observed when ∼ 50% Mg2+ is discharged is retarded by bongkrekate and added Mg2+ whereas it is accelerated by atractylate. All adenine nucleotides have a significant effect in retarding the efflux of accumulated Ca2+ but, in the presence of oligomycin and P1,P5-di(adenosine-5′)-pentaphosphate only ADP is active. From these results we conclude that effluxes of Mg2+, Ca2+ and adenine nucleotide from rat liver mitochondria induced by external phosphate are interconnected and regulated by external ADP and Mg2+ levels.This publication has 19 references indexed in Scilit:
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