Lipid Metabolism in Non-Uremic and Uremic Dogs During and After Hemodialysis with Acetate

Abstract

Plasma triglyceride and cholesterol concentrations were elevated in dogs after nephrectomy induced uremia. Plasma triglyceride concentrations remained constant during a 4-hour period of hemodialysis of uremic and non-uremic dogs against an acetate concentration of 39. 5 mM (delivering 3 mEq/Kg/hr) plus infusion of 12. 5 µ;Ci/Kg/hr of acetate-1-14C, but rose progressively following dialysis. Radioactivities in plasma phospholipids, triglycerides and cholesteryl esters increased during dialysis and continued to rise in the post dialysis period, whereas 14C activity in free fatty acids and free cholesterol increased during dialysis and decreased post-dialysis. Acetate 14-C incorporation into plasma triglycerides was similar in the uremic and non-uremic groups, but incorporation into plasma cholesterol was higher in the uremics. Liver triglyceride concentrations and radioactivities at 12 hours were higher in the uremic dogs. At this time, adipose tissue 14C incorporation was also higher in uremic dogs but no differences were observed in aorta, heart or sciatic nerve incorporation. The results suggest that acetate may contribute to the increased plasma triglyceride concentrations observed following dialysis, and that uremia further accentuates acetate incorporation into plasma cholesterol and liver triglycerides.