Long‐term maintenance of “cloned” human PLT cells in TCGF with LCL cells as a feeder layer
- 1 January 1980
- journal article
- research article
- Published by Wiley in Journal of Supramolecular Structure
- Vol. 13 (4), 525-532
- https://doi.org/10.1002/jss.400130411
Abstract
The long-term maintenance of T cells “cloned” by limiting dilution in TCGF was enhanced by the use of irradiated autologous lymphoblastoid cell line (LCL) cells as well as irradiated LCL cells of the individual to which the T cells were originally primed. It was possible to obtain more than 1 × 1012 cells from a “clone” seeded at one cell per well. Some of the clones tested express primed LD-typing activity.Keywords
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