Leukoagglutination Differentiation of Normal Leukocytic and Leukemic Cell-Types

Abstract

In a previous publication the production of antileukocytic sera and a method of agglutinating leukocytes were described (1). In a later communication we presented a more rapid method of leukoagglutination and titration and indicated some of the environmental factors that may influence the reaction (2). The present study is concerned with determining the specificity of the cell-antigens of the various types of leukocytes obtained from normal and leukemic people. Materials and Methods. Six antileukocytic sera were produced in rabbits (1). The antigens were leukocytes obtained from individuals with acute and chronic myelocytic leukemia, acute and chronic lymphocytic leukemia, normal people (leukocytes from blood-bank), and normal lymph-nodes. The total peripheral-leukocyte count in the patient with acute myelocytic leukemia was 621,000 cells per mm³ of blood with the following distribution in per cent: 77 blasts, 3.5 myelocytes and metamyelocytes, 19 juveniles and polylobed neutrophilic polymorphonuclear leukocytes, and 0.2 eosinophilic and basophilic granulocytes.