Carbohydrate Metabolism in Agaricus bisporus: Oxidative Pathways in Mycelium and Sporophore

Abstract

The relative rates of the glycolytic pathways were compared in vegetative mycelium and sporophore tissue of A. bisporus. Simple radiorespirometry gave a C-I/C-6 ratio of 8.3 for pileus slices and 4.6 for vegetative mycelium. Glucose-6-phosphate dehydrogenase [EC 1.1.1.49] activity was greater in sporophores than in vegetative mycelium; glucosephosphate isomerase [EC 5.3.1.9] activity was higher in the mycelium. There was no significant difference between mannitol dehydrogenase activities in sporophore and mycelium. The results were consistent with a greater proportion of glucose oxidation occurring via the hexose monophosphate pathway in the sporophore than in the mycelium.