The use of bacteriophage neutralization technique for the detection of anti-hapten antibody

Abstract

The phage neutralization technique was used for the detection of rabbit antibodies against the 2,4-dinitrophenyl residue. The Na Salt of di-nitrobenzene sulfonate was allowed to react with bacteriophage T2 and the surviving hapten-bacteriophage conjugates (HBC) found to be inactivated by the anti-hapten antiserum. Amounts of antibody as low as 2 x 10-3[mu]g were detected by this technique. The specificity of the reaction was demonstrated by the inhibition with structurally related haptens of the neutralization of HBC by anti-hapten antibody.