In vitro Culture of White Clover: Callus, Suspension, Protoplast Culture, and Plant Regeneration
- 1 June 1980
- journal article
- research article
- Published by University of Chicago Press in Botanical Gazette
- Vol. 141 (2), 157-164
- https://doi.org/10.1086/337138
Abstract
Callus and suspension cultures of T. repens (white clover) were established from seedlings. Protoplasts, isolated enzymatically from cell aggregates of suspension cultures, produced callus which was capable of in vitro differentiation into complete plants. Regenerated plants were nodulated if inoculated with Rhizobium trifolii. Karyotypic stability (2n = 4x = 32) was maintained for 2 yr of callus/suspension culture and through organogenesis. Suspension cultures were treated enzymatically to give single-cell populations capable of high plating efficiencies is soft-agar media.Keywords
This publication has 2 references indexed in Scilit:
- Studies on the Organogenesis in Carrot Tissue Cultures II. Effects of Amino Acids and Inorganic Nitrogenous Compounds on Somatic EmbryogenesisZeitschrift für Pflanzenphysiologie, 1979
- Callus and Plantlet Regeneration from Cell Cultures of Ladino Clover and SoybeanPhysiologia Plantarum, 1977