THE FATE OF TRANSFORMING DEOXYRIBONUCLEATE FOLLOWING FIXATION BY TRANSFORMABLE BACTERIA, III

Abstract

A strepto-mycin-resistant strain of pneumococcus was grown on a medium containing p32 as orthophosphate. Excess inorganic phosphate and bacteria were added to this. After a period of chilling, the DNA was isolated by lysis and exposed to a transformable population of pneumococcus. DNase was added, the culture was incubated, and these cells were lysed. The resulting DNA was called reisolated DNA. There was little difference between the rates at which the p32 in the original and reisolated DNA preparations lost their biological activity.