Evaluation of a Simplified Microchromatographic Technique for Hemoglobin A2 Determination
- 1 January 1979
- journal article
- research article
- Published by S. Karger AG in Acta Haematologica
- Vol. 61 (1), 15-26
- https://doi.org/10.1159/000207621
Abstract
The simplified Hb A2 determination based on microchromatography in Pasteur pipets filled with DEAE-cellulose with glycine-KCN-NaCl as developers [14] is compared with a reference Hb A2 determination procedure based on starch-block electrophoresis. The utility of microchromatography as a routine Hb A2 assay and as a screening method to detect β-thalassemia trait carriers and patients with iron deficiency anemia was investigated. Day-to-day variation of a control hemolysate and the correlation between the values obtained with the two methods and between determinations in duplicate on the same sample are given.Keywords
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