Localization of the synthesis of very-long-chain fatty acid in mitochondria from Saccharomyces cerevisiae
- 1 October 1988
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 177 (1), 207-211
- https://doi.org/10.1111/j.1432-1033.1988.tb14363.x
Abstract
The localization of the mitochondrial elongation activities (''elongases'') from Saccharomyces cerevisiae has been investigated. It was shown, using carboxyatractyloside in the incubation mixture, that synthesis of very-long-chain fatty acids probably occurred outside the matrix and, by fractionation experiments, that elongases are membrane-bound enzymes. The solubilization of the outer membrane by digitonin showed that three elongating activities are correlated with a marker of the outer membrane and not with an inner membrane marker. A further partial purification of the outer membrane showed that elongases are present in the outer membrane of mitochondria.This publication has 15 references indexed in Scilit:
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