Identification and Origin of Some Chloroplast Aminoacyl-tRNA Synthetases and tRNAs
- 1 November 1970
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 67 (3), 1207-1213
- https://doi.org/10.1073/pnas.67.3.1207
Abstract
Light-grown, wild type Euglena gracilis contains two aminoacyl-tRNA synthetases for both phenylalanine and isoleucine. Only one of the two synthetases for each amino acid is found in isolated chloroplasts, as are the light-induced phenylalanine and isoleucine tRNAs. In each case the light-induced chloroplast tRNAs can only be acylated by the chloroplast synthetases. The chloroplast isoleucyl-tRNA synthetase is light-inducible and cannot be detected in dark-grown cells or in cells of the bleached mutant W(3)BUL. The presence of the chloroplast phenylalanyl-tRNA synthetase in W(3)BUL, which contains no chloroplast DNA or structure, indicates that this chloroplast enzyme is synthesized in the cytoplasm and is coded by nuclear genes.Keywords
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