Effect of chlorhexidine on the release of lysosomal enzymes from cultured macrophages
- 1 January 1981
- journal article
- research article
- Published by Taylor & Francis in Acta Odontologica Scandinavica
- Vol. 39 (5), 285-289
- https://doi.org/10.3109/00016358109162291
Abstract
Exposure of rat's peritoneal macrophages to chlorhexidine at concentrations up to 0.01 per cent resulted in the release of lysosomal enzymes, e.g. chloride-dependent arginine aminopeptidase and β-D-glucuronidase into the medium from resting cells. The activity of extracellular lactate dehydrogenase increased up to the concentration of 0.01 % and then decreased with increasing chlorhexidine concentration. This could indicate a certain type of »mummifying« of the cells and/or their membranes. The incorporation of trypan blue was found in all cells after a rinse with 0.05 per cent or higher concentrations of chlorhexidine. The direct effect of chlorhexidine on these enzyme activities showed a slight activation of β-D-glucuronidase and lactate dehydrogenase, but an inhibition of arginine aminopeptidase. Also the binding of chlorhexidine to cells was found. Low chlorhexidine concentrations caused an additional enzyme release from phagocytizing cells.Keywords
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