High resolution 31P nuclear magnetic resonance studies of intact yeast cells.
Open Access
- 1 December 1975
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (12), 4966-4970
- https://doi.org/10.1073/pnas.72.12.4966
Abstract
High resolution 31P nuclear magnetic resonance (NMR) spectra at 145.7 MHZ are presented for intact yeast cells. Several peaks are resolved and assigned. They include the middle phosphate peaks from long chain or cyclic polyphosphates. Our results are consistent with the suggestion that these polyphosphates act as a phosphate store in the cell. We have also been able to measure cytoplasmic pH using the orthophosphate peak inside the cell, as compared with outside the cell. The results show that yeast cells maintain their cytoplasmic pH around 6.3. This value is considerably higher than the acidic extracellular pH at which they normally live. These preliminary results indicate that 31P NMR at 145.7 MHZ can be a rapid, informative, and non-invasive method for probing biochemical events within living cells.Keywords
This publication has 6 references indexed in Scilit:
- 31P magnetic resonance of tRNA.Proceedings of the National Academy of Sciences, 1975
- Observation of tissue metabolites using 31P nuclear magnetic resonanceNature, 1974
- Phosphate Metabolism in Intact Human Erythrocytes: Determination by Phosphorus-31 Nuclear Magnetic Resonance SpectroscopyProceedings of the National Academy of Sciences, 1974
- Determination of Intracellular pH by 31P Magnetic ResonanceJournal of Biological Chemistry, 1973
- Inorganic polyphosphates in biology: structure, metabolism, and function.1966
- Interactions of Arsenate with the Phosphate-Transporting System of YeastThe Journal of general physiology, 1963