Autoimmune antigen Ku is enriched on oligonucleotide columns distinct from those containing the octamer binding protein DNA consensus sequence
- 29 July 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 286 (1-2), 225-228
- https://doi.org/10.1016/0014-5793(91)80979-d
Abstract
During purification of the AP1 complex from the T cell line MLA144 we enriched for a complex which bound to an oligonucleotide column containing the AP1 DNA consensus sequence and co-eluted with a fraction required for AP1 binding activity. This complex although co-eluting with AP1 binding activity had previously been determined to be non-specific in its DNA binding properties. Further investigation determined that the complex was a heterodimer of 85 and 70 kDa which was antigenically related to the autoimmune antigen Ku. It is important to be aware of the abundance and avidity of the Ku complex to bind oligonucleotide columns when purifying sequence specific binding proteins.Keywords
This publication has 16 references indexed in Scilit:
- The human U1 snRNA promoter correctly initiates transcription in vitro and is activated by PSE1.Genes & Development, 1990
- Cell surface expression of the 70-kD component of Ku, a DNA-binding nuclear autoantigen.JCI Insight, 1990
- Multiple components are required for sequence recognition of the AP1 site in the gibbon ape leukemia virus enhancer.Molecular and Cellular Biology, 1989
- Transcriptional Regulation in Mammalian Cells by Sequence-Specific DNA Binding ProteinsScience, 1989
- Binding of a cellular protein to the gibbon ape leukemia virus enhancer.Molecular and Cellular Biology, 1987
- Characterization of the DNA-binding protein antigen Ku recognized by autoantibodies from patients with rheumatic disorders.Journal of Biological Chemistry, 1986
- A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genesNature, 1986
- A protein binds to a satellite DNA repeat at three specific sites that would be brought into mutual proximity by DNA folding in the nucleosomeCell, 1984
- [36] Eukaryotic gene transcription with purified componentsMethods in Enzymology, 1983
- Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresisNucleic Acids Research, 1981