Acrosome reaction in the head-attached human sperm
- 24 April 2009
- journal article
- Published by Hindawi Limited in Andrologia
- Vol. 26 (1), 17-20
- https://doi.org/10.1111/j.1439-0272.1994.tb00747.x
Abstract
The physiological acrosome reaction of the sperm occurs on the surface of the oocyte. Although its occurrence on the zona pellucida is considered to be a specific response to zona compounds, whether the simple attachment itself could influence the reaction of motile spermatozoa to a fixed surface and promote onset of the reaction is questioned. A motile human sperm head fixation method was developed recently, through which a group of head-attached sperm can be established easily. These head-attached sperm were investigated for their acrosome reactions. After staining by fluorescein iso-thiocyanate conjugated peanut lectin (FITC-PNA), many head-attached spermatozoa (75.7%) were found to have undergone an acrosome reaction.Keywords
This publication has 15 references indexed in Scilit:
- A motile human sperm head fixation methodAndrologia, 2009
- Effect of multiple centrifugations on the evaluation of the acrosome reaction in human spermatozoaAndrologia, 2009
- Sperm/egg binding patterns and oocyte cytology in retrospective analysis of fertilization failure in vitroHuman Reproduction, 1993
- High progesterone concentrations induce acrosome reaction with a low cytotoxic effectFertility and Sterility, 1992
- Inhibition of the human sperm acrosome reaction by a high molecular weight factor from human seminal plasma**Supported by grant no. HD 19555 from the National Institutes of Health, Bethesda, Maryland.††Presented at the 22nd Annual Meeting of the Society for the Study of Reproduction, Columbia, Missouri, August 6 to 9, 1989.Fertility and Sterility, 1990
- Extended exposure to follicular fluid is required for significant stimulation of the acrosome reaction in human spermatozoaReproduction, 1989
- The spontaneous acrosome reaction of human spermatozoa incubated in vitroHuman Reproduction, 1989
- Induction of Acrosome Reactions by the Human Zona Pellucida1Biology of Reproduction, 1988
- Early Penetration of Human Sperm Through the Vestments of Human Eggs in VitroArchives of Andrology, 1986
- Penetration of Human Spermatozoa into the Human Zona Pellucida and the Zona-Free Hamster Egg: A Study of Fertile Donors and Infertile PatientsFertility and Sterility, 1980