PHYSIOLOGY OF WOOD-ROTTING BASIDIOMYCETES: IV. RESPIRATION OF NON-PROLIFERATING CELLS OF POLYPORUS PALUSTRIS
- 1 February 1962
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 8 (1), 145-156
- https://doi.org/10.1139/m62-019
Abstract
For manometric studies of respiration, small, whole mycelial pellets of Polyporus palustris grown in submerged (shake) culture were much superior to fragmented mycelium. The rates of endogenous and of exogenous respiration were dependent, in part, upon age of culture, and were maximal at pH 5.5. The endogenous respiration was high but could be reduced by starvation (aeration in a non-nutrient medium), permitting measurement of exogenous respiration upon the addition of oxidizable substrate. Of the carbohydrates studied, xylose, glucose, galactose, and sucrose were the most stimulative to aerobic respiration; rhamnose, melezitose, and lactose either were not oxidized or showed only slight stimulation. The alcohols methanol, ethanol, glycerol, and propylene glycol were readily utilized; inositol was oxidized to a lesser degree. The sugar alcohols mannitol, sorbitol, and adonitol were mildly stimulative; the effect of dulcitol was dependent upon its concentration. The fatty acids acetate and caprylate were much more readily utilized than butyrate. Salts of other acids— oxalate, pyruvate, lactate, and fumarate—were oxidized to about the same extent as butyrate. Non-proliferating cells of P. palustris had a definite anaerobic metabolism and fermented glucose with production of carbon dioxide and small amounts of acid(s). In air, oxidative assimilation of glucose was high (79–91%); endogenous respiration apparently was not suppressed during assimilation.Keywords
This publication has 18 references indexed in Scilit:
- Respiration of Amino Acids by Streptomyces nitrificansMycologia, 1956
- Physiology of the Wood-Rotting Basidiomycetes. I. Growth and Nutrition in Submerged Culture in Synthetic MediaMycologia, 1955
- MICROBIAL METABOLISM OF CARBAMATESJournal of Bacteriology, 1955
- Carbohydrate Metabolism and Oxalic Acid Synthesis by Botrytis cinerea.Plant Physiology, 1954
- FACTORS AFFECTING STREPTOMYCIN YIELDSAmerican Journal of Botany, 1953
- THE METABOLISM OF SPECIES OF STREPTOMYCES VJournal of Bacteriology, 1952
- BIOCHEMISTRY OF FILAMENTOUS FUNGI IJournal of Bacteriology, 1951
- STUDIES OF THE RESPIRATION OF THE MYCELIUM OF THE FUNGUS MYROTHECIUM VERRUCARIAAmerican Journal of Botany, 1950
- RELATION OF OXYGEN PRESSURE AND TEMPERATURE TO GROWTH AND CARBON‐DIOXIDE PRODUCTION IN THE FUNGUS POLYSTICTUS VERSICOLORAmerican Journal of Botany, 1937
- The oxidative metabolism of the colorless alga, Prototheca zopfiiJournal of Cellular and Comparative Physiology, 1936