Determination of microsomal UDP-glucuronyltransferase in needle-biopsy specimens of human liver

Abstract

Sensitive, reliable and convenient assays are described for the study of human liver microsomal UDP-glucuronyltransferase. Using¹⁴C-1-naphthol as substrate about 2 mg of a liver biopsy specimen and for¹⁴C-morphine about 20 mg of tissue will suffice for enzyme estimation. Lack of inhibition of 1-naphthol glucuronidation by morphine suggests that the substrates are glucuronidated by different forms of the enzyme. Enzyme levels in the native and activated state were studied in biopsies from patients grouped according to histopathological and clinical criteria. The enzyme assays may help to characterize UDP-glucuronyltransferases in human tissues and their induction by drugs and environmental chemicals, as well as their alteration in various diseases.