Products of Orthophosphate Absorption by Barley Roots

Abstract
Eighty to 90% of the orthophosphate absorbed by excised barley roots in 15 seconds to 2 hours is incorporated into 5 compounds. Kinetic studies show that all 5 are products of the rate-limiting absorption step. The earliest compounds in sequence of incorporation are uridine diphosphate glucose (UDPG), glucose-1-phosphate (G-l-P) and inorganic phosphate (Pi). Four of the compounds, UDPG, G-l-P and 2 unknowns arise from absorption through 1 site which appears to be linked to the cytochrome b site of phosphorylation in the respiratory chain. Pi is a product of absorption through another site and is apparently linked to diphosphopyridine nucleotide.