Spore Germination and Carbon Metabolism in Fusarium solani V. Changes in Anaerobic Metabolism and Related Enzyme Activities during Development

Abstract

Macroconidia of Fusarium solani f. phaseoli had no detectable capacity for anaerobic respiration, a capacity present in germinated spores and mycelium. Measurements of the specific activity of the known enzymes of the glycolytic pathway showed that all were present in ungerminated spores; hence, the acquisition of fermentative capacity during germination was not the result of the synthesis of enzymes not present in the ungerminated spore. Most enzyme specific activities increased during development from spore to mycelium, and the rate of fermentation appeared to be limited by factors other than the enzymes tested (hexokinase, phosphofructokinase, adolase, triose phosphate isomerase, triose phosphate dehydrogenase, phosphoglyceric kinase, phosphoglyceric mutase, enolase, pyruvate kinase, and pyruvate decarboxylase).