Survival of Escherichia coli in freshwater: β-D-Glucuronidase activity measurements and characterization of cellular states
- 1 July 2000
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 46 (7), 679-684
- https://doi.org/10.1139/w00-040
Abstract
A rapid enzyme assay measuring β-D-glucuronidase activity of Escherichia coli was tested in survival experiments after discharge of E. coli in river water. Enzyme activity was compared with several analyses performed to characterize cellular states under stressful conditions. Enzyme activity remained stable under starvation and light stress conditions despite losses of culturability, respiratory activity, and cytoplasmic membrane integrity. β-D-Glucuronidase activity followed the pattern of genetic and morphologic cell integrity. The tested enzyme assay seems well adapted to study the fate of fecal coliforms in survival experiments, and appears to be a rapid and efficient way to estimate the microbiological quality of surface waters.Key words: β-D-glucuronidase activity, Escherichia coli, survival, freshwater, cellular states.Keywords
This publication has 17 references indexed in Scilit:
- LIVE/DEAD® BacLight™: application of a new rapid staining method for direct enumeration of viable and total bacteria in drinking waterJournal of Microbiological Methods, 1999
- Survival of allochthonous bacteria in aquatic systems: a biological approachFEMS Microbiology Ecology, 1997
- Succession of cellular states in a Salmonella typhimurium population during starvation in artificial seawater microcosmsFEMS Microbiology Ecology, 1997
- Effect of seawater on Escherichia coli β‐galactosidase activityJournal of Applied Bacteriology, 1996
- β-D-galactosidase activity of viable, non-culturable coliform bacteria in marine watersLetters in Applied Microbiology, 1995
- Detection of induced β‐galactosidase activity in individual non‐culturable cells of pathogenic bacteria by quantitative cytological assayMolecular Microbiology, 1995
- Estimation ofEscherichia coli mortality in seawater by the decrease in3H-label and electron transport system activityMicrobial Ecology, 1989
- Viability and virulençe ofEscherichia colisuspended by membrane chamber in semitropical ocean waterFEMS Microbiology Letters, 1986
- Viable but nonrecoverable stage of Salmonella enteritidis in aquatic systemsCanadian Journal of Microbiology, 1984
- The use of DAPI for identifying and counting aquatic microflora1Limnology and Oceanography, 1980