Abstract
The numbers of .alpha.-naphthyl acetate esterase (ANAE)-containing cells (other than T lymphocytes) in non-Hodgkin''s lymphomas (NHL) and reactive lymph nodes were counted, using the Reichert-Jung (Kontron) MOP-AMO3, user-controlled image analyzer. Twenty [human] specimens of NHL and 10 reactive nodes were examined. Cells were demonstrated by their content of acid .alpha.-naphthyl acetate esterase (ANAE) in fixed frozen sections. Lymphomas of high-grade malignancy contained much larger numbers of ANAE-positive cells (10.8-20.5%) than those of low-grade malignancy (1.4-4.1%). The number of ANAE-positive cell (1.4-3.2%) in reactive lymph nodes was similar to that in low-grade NHL nodes.