Receptor Mobility and the Mechanism of Cell-Cell Binding Induced by Concanavalin A
- 1 June 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (6), 2456-2460
- https://doi.org/10.1073/pnas.71.6.2456
Abstract
The cell-cell binding induced by concanavalin A between single cells has been analyzed by use of cells attached to nylon fibers. Binding of a concanavalin A-coated cell to an untreated cell was found to a high degree between two lymphoma tumor cells, less frequently between a lymphoma cell and a normal lymphocyte, and only rarely between two normal lymphocytes. The binding was inhibited by the presence of a saccharide inhibitor of concanavalin A, but could not be reversed by addition of the inhibitor after the cells had bound to each other. Although no binding was obtained when both cells were coated with lectin or fixed with glutaraldehyde, fixation of a cell before coating with concanavalin A enhanced its ability to bind an untreated cell. The results indicate that cell-cell binding induced by concanavalin A requires short-range lateral movement of cell receptors for the lectin, that only one cell has to have mobile receptors, and that some receptors must be unoccupied by lectin molecules before cell-cell contact. Clustering of the receptors is not necessary and seems to hinder cell-cell binding. It is suggested that the short-range movement is required for alignment of individual receptors so as to form multi-point bridges between two cells by lectin molecules. The bridging is then followed by the formation of irreversible bonds between the cells. The receptors on tumor cells appear to have a greater ability than receptors on normal cells to align themselves for cell-cell binding.Keywords
This publication has 17 references indexed in Scilit:
- THE RELATIONSHIP OF CONCANAVALIN A BINDING TO LECTIN-INITIATED CELL AGGLUTINATIONThe Journal of cell biology, 1973
- Ligand-induced Redistribution of Concanavalin a Receptors on Normal, Trypsinized and Transformed FibroblastsNature New Biology, 1973
- Inhibition of lectin agglutinability by fixation of the cell surface membraneBiochimica et Biophysica Acta (BBA) - Biomembranes, 1973
- Membrane Changes and Adenosine Triphosphate Content in Normal and Malignant Transformed CellsProceedings of the National Academy of Sciences, 1973
- A Comparative Evaluation of the Distribution of Concanavalin A-Binding Sites on the Surfaces of Normal, Virally-Transformed, and Protease-Treated FibroblastsProceedings of the National Academy of Sciences, 1973
- Temperature-dependent Mobility of Concanavalin a Sites on Tumour Cell SurfacesNature New Biology, 1973
- Mobility of carbohydrate containing sites on the surface membrane in relation to the control of cell growthFEBS Letters, 1973
- The mechanism of cell fusion. I. Energy requirements for virus-induced fusion of Ehrlich ascites tumor cells.1972
- Specific Fractionation of Immune Cell PopulationsProceedings of the National Academy of Sciences, 1972
- Cell Fractionation and Arrangement on Fibers, Beads, and SurfacesProceedings of the National Academy of Sciences, 1971