Effects of methadone on ornithine decarboxylase and cyclic nucleotide phosphohydrolase in neuronal and glial cell cultures
- 1 January 1982
- journal article
- research article
- Published by Wiley in Journal of Neuroscience Research
- Vol. 7 (2), 111-117
- https://doi.org/10.1002/jnr.490070203
Abstract
Mixed neuronal and nonneuronal cell cultures were obtained from 8‐day‐old chick embryos cerebral hemispheres and glial‐enriched cultures were obtained from fifteen‐day‐old chick embryo cerebral hemispheres. Cultures were exposed to methadone, a narcotic drug, from days four to six. The activity of ornithine decarboxylase (ODC) was determined at day eight and the activity of cyclic nucleotide phosphohydrolase (CNP) was determined at day fifteen. Both ODC and CNP activity were higher in mixed neuronal‐nonneuronal cell cultures treated with methadone as compared to control. No effect was observed in the neuronal‐enriched or glial‐enriched cultures. These findings are interpreted to reflect that neuronal‐glial interaction is important in the response of primary neural cells to methadone.Keywords
This publication has 26 references indexed in Scilit:
- Role of Polyamines in the Control of Cell Proliferation and DifferentiationDifferentiation, 1981
- Morphine-induced striatal dopamine efflux depends on the activity of nigrostriatal dopamine neuronesNature, 1979
- Effects of 4-aminopyridine and tetraethylammonium on the depolarization by GABA of cultured satellite glial cellsNeuroscience Letters, 1979
- Levels of ornithine decarboxylase activation used as a simple marker of metal induced growth arrest in tissue cultureLife Sciences, 1979
- Functional interactions between neurons and astrocytes I. Turnover and metabolism of putative amino acid transmittersProgress in Neurobiology, 1979
- Stimulation of non-neuronal cell proliferation in vitro by mitogenic factors present in highly purified sympathetic neuronsBrain Research, 1978
- Some factors influencing the proliferation and differentiation of myelin‐forming cellsNeurology, 1978
- Evidence that contact with connective tissue matrix is required for normal interaction between Schwann cells and nerve fibers.The Journal of cell biology, 1978
- ORNITHINE AND GLUTAMIC ACID DECARBOXYLASE ACTIVITIES IN THE DEVELOPING CHICK RETINAJournal of Neurochemistry, 1976
- ORNITHINE DECARBOXYLASE ACTIVITY IN DEVELOPING RAT BRAIN1Journal of Neurochemistry, 1972