Preservation of Human Erythrocytes in the Liquid State: Biological Results with a New Medium

Abstract

Red blood cells (RBC) were collected with citrate-phosphate-dextrose (CPD) in a blood-pack optimal additive system. After concentration to 90% hematocrit they were diluted with saline-adenine-glucose medium (SAG-RBC) and stored for 35 days. The RBC were stored in the presence of leukocytes. The SAG medium allows RBC conservation during 35 days at +4.degree. C. The ATP level of RBC is compatible with their survival. During the first 2 wk, hemolysis of SAG-RBC was not greater than in CPD blood. Hemolysis reached 1.49% on day 35, and there was a marked increase in RBC osmotic fragility. Scanning electron microscopic studies of 35-day RBC showed that the majority of them became echinocytes. After incubation in fresh frozen plasma, the RBC recovered satisfactory osmotic resistance and normal disc shape. The post-transfusion viability was normal with > 70% recovery after 48 h. The in vivo restoration of 2,3-diphosphoglycerate (2,3-DPG) was rapid in the transfused SAG-RBC, 50% of the initial 2,3-DPG level being restored in 1 h. The in vivo studies proved that the functional quality of these RBC was compatible with their use in transfusion. The most important problem concerns the supernatant Hb level of the SAG-RBC to be used for massive transfusion.