Limitations Due to Unstirred Layers in Measuring Channel Response of Excised Membrane Patch Using Rapid Solution Exchange Methods.

Abstract

The Ca(2+)-activated K+ channel was activated by a step increase in the intracellular Ca2+ concentration ([Ca2+]i) using the oil-gate concentration jump method in hippocampal neurons of rat. Limitations due to the unstirred volume between the excised inside-out membrane and the tip opening were examined.