Effects of capsaicin on rat liver S9‐mediated metabolism and DNA binding of aflatoxin
- 1 January 1991
- journal article
- other
- Published by Taylor & Francis in Nutrition and Cancer
- Vol. 15 (1), 27-32
- https://doi.org/10.1080/01635589109514108
Abstract
At concentrations of 25, SO, and 100 μM, capsaicin, which is the major component in various aspects of Capsicum hot peppers, decreased the binding of aflatoxin (AFB1) to calf thymus DNA by 19%, 44%, and 71%, respectively, in incubations with rat liver S9. At concentrations of 50 and 100 μM, capsaicin decreased the formation of AFB‐DNA adducts (AFB1‐N7‐Gua) by 53% and 75% as determined by high‐pressure liquid chromatography (HPLC). HPLC analysis of organosoluble fractions showed that these effects correlated with a concentration‐dependent decrease in S9‐mediated metabolism of AFB, by capsaicin. Capsaicin also altered the formation of water‐soluble conjugates of AFB1. This was indicated by a decrease in radioactivity in water‐soluble fractions and in glutathione conjugates of AFB1 analyzed by HPLC. These results suggest that capsaicin inhibited the biotransformation of AFB1 by modifying Phase I hepatic enzyme activity.Keywords
This publication has 10 references indexed in Scilit:
- Comparative effects of butylated hydroxyanisole on hepatic in vivo DNA binding and in vitro biotransformation of aflatoxin B1 in the rat and mouseToxicology and Applied Pharmacology, 1987
- Inhibition of aflatoxin B1 mutagenesis in Salmonella typhimurium and DNA damage in cultured rat and human tracheobronchial tissues by ellagic acidCarcinogenesis: Integrative Cancer Research, 1987
- Action of intrathecal capsaicin and its structural analogues on the content and release of spinal substance P: Selectivity of action and relationship to analgesiaBrain Research, 1984
- Capsaicin: hot new pharmacological toolTrends in Pharmacological Sciences, 1983
- Revised methods for the Salmonella mutagenicity testMutation Research/Environmental Mutagenesis and Related Subjects, 1983
- Interaction of capsaicinoids with drug-metabolizing systems. Relationship to toxicityBiochemical Pharmacology, 1983
- Metabolic Activation of Aflatoxin B1: Patterns of DNA Adduct Formation, Removal, and Excretion in Relation to CarcinogenesisDrug Metabolism Reviews, 1982
- Comparative mutagenicity of aflatoxins using a Salmonella/trout hepatic enzyme activation systemCarcinogenesis: Integrative Cancer Research, 1982
- Differences in aflatoxin B1-susceptibility of rat and mouse are correlated with the capability in vitro to inactivate aflatoxin B1- epoxideCarcinogenesis: Integrative Cancer Research, 1981
- The role of aflatoxin metabolism in its toxic lesionToxicology and Applied Pharmacology, 1976