A sensitive and efficient isoenzyme technique for small arthropods and other invertebrates
- 1 June 1987
- journal article
- research article
- Published by Cambridge University Press (CUP) in Bulletin of Entomological Research
- Vol. 77 (3), 407-415
- https://doi.org/10.1017/s0007485300011871
Abstract
An electrophoretic method for the study of enzyme variation, which uses cellulose acetate sheets with an agar overlay for staining, the use of a very good general purpose buffer (citric-aminopropyldiethanol amine) and the use of sodium azide as a bacteriocide to allow long term storage of chemicals as solutions are described. Tests are reported of the technique onTetranychus urticaeKoch,Aedes aegypti(L.) and several species ofDrosophila. The results demonstrate that the technique offers sensitivity equal to or greater than starch or polyacrylamide gel electrophoresis and that it is applicable to very small organisms, allowing either the testing of single individuals for large numbers of enzymes or the testing of fewer enzymes under different electrophoretic conditions (i.e. to detect cryptic variation under a single condition). The technique is efficient of time and materials, and safer than conventional methods.This publication has 29 references indexed in Scilit:
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