METABOLISM OF CARBOHYDRATES BY PSEUDOMONAS SACCHAROPHILA I

Abstract

Three types of mutants of P. saccharophila can be differentiated from the parent strain by their ability to grow with glucose, fructose, or either hexose as substrate. The ability to oxidize fructose by fructose-utilizing strains is conditioned by the growth substrate and by the physiological state of the cells. Glucose and certain other carbohydrates have a strong inhibitory effect on fructose oxidation. Evidence is presented for phosphorylation of fructose and its subsequent oxidation via the 2-keto, 3-deoxy, 6-phosphogluconic acid pathway.