Slow passive diffusion of NAD+ between intact isolated plant mitochondria and suspending medium
- 15 November 1983
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 216 (2), 443-450
- https://doi.org/10.1042/bj2160443
Abstract
Isolated potato (Solanum tuberosum) tuber mitochondria purified by isopycnic centrifugation in density gradients of Percoll were highly intact, devoid of extramitochondrial contaminations and retained a high rate of O2 consumption. When suspended in a medium that voided rupture of the outer membrane, intact purified mitochondria progressively lost their NAD+ content by passive diffusion. This led to a slow decrease of oxoglutarate-dependent O2 consumption by isolated mitochondria. Addition of NAD+ to the medium restored the initial state-3 rate of oxoglutarate oxidation. The rate of NAD+ accumulation in the matrix space was concentration-dependent, exhibited Michaelis-Menten kinetics and was strongly inhibited by the analog N-4-azido-2-nitrophenyl-4-aminobutyryl-NAD+.This publication has 21 references indexed in Scilit:
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