Gas Chromatography of Methyl Thiohydanmins of Amino Acids

Abstract

The methyl thiohydantoins of twenty-two amino acids commonly encountered in protein sequence work were prepared and their behavior on gas chromatography investigated. Sixteen of these were separated from each other by the use of two columns with different silicone stationary phases. Five of the remaining ones, the methyl thiohydantoins of aspartic acid, serine, arginine, carboxymethyl cysteine, and cysteic acid, which gave decomposition and a common peak in the above systems, gave unique peaks following silylation. The methyl thiohydantoin of cysteine was not successfully analyzed.