Transformation and transfection in lysogenic strains of Bacillus subtilis: evidence for selective induction of prophage in competent cells
- 1 January 1975
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 121 (1), 296-304
- https://doi.org/10.1128/jb.121.1.296-304.1975
Abstract
Lysogenic strains of Bacillus subtilis 168 were reduced in their level of transformation as compared to non-lysogenic strains. The level of transformation decreased even further if the competent lysogenic cells were allowed to incubate in growth media prior to selection on minimal agar. This reduction in the frequency of transformation was attributable to the selective elimination of transformed lysogenic cells from the competent population. Concurrent with the decrease in the number of transformants from a lysogenic competent population was the release of bacteriophage by these cells. The lysogenic bacteria demonstrated this dramatic release of bacteriophage only if the cells were grown to competence. Both the selective elimination of transformed lysogens and the induction of prophage was prevented by the inhibition of protein synthesis. Additionally, competent lysogenic cells released significantly higher amounts of exogenous donor transforming deoxyribonucleic acid than did competent non-lysogenic cells or competent lysogenic cells incubated with erythromycin. These data establish that the induction of the prophage from the competent lysogenic cells was responsible for the selective elmination of the lysogenic transformants. A model is presented that accounts for the induction of the prophage from competent lysogenic bacteria via the induction of a repair system. It is postulated that a repair system is induced or derepressed by the accumulation of gaps in the chromosomes of competent bacteria. This hypothetical enzyme(s) is ultimately responsible for the induction of the prophage and the selective elimination of transformants.Keywords
This publication has 55 references indexed in Scilit:
- RECOMBINATION DEFICIENT MUTANTS OF E. COLI AND OTHER BACTERIAAnnual Review of Genetics, 1973
- The influence of temperate bacteriophage φ105 on transformation and transfection in BacillussubtilisBiochemical and Biophysical Research Communications, 1972
- Fate of transforming DNA following uptake by competent Bacillus subtilis: III. Formation and properties of products isolated from transformed cells which are derived entirely from donor DNAJournal of Molecular Biology, 1972
- Inhibitors of Ribosome FunctionsAnnual Review of Microbiology, 1971
- Inhibition of bacterial DNA replication by 6-(p-Hydroxyphenylazo)-uracil: Differential effect on repair and semi-conservative synthesis in Bacillus subtilisJournal of Molecular Biology, 1971
- RNA POLYMERASEAnnual Review of Biochemistry, 1971
- Ultraviolet reactivation and ultraviolet mutagenesis of λ in different genetic systemsVirology, 1971
- Some properties of DNA in competent Bacillus subtilisJournal of Molecular Biology, 1969
- Recombination and DNA replication in Bacillus subtilis transformationMutation Research, 1968
- TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATEProceedings of the National Academy of Sciences, 1958