Anatomical substrates of cholinergic‐autonomic regulation in the rat
- 1 February 1990
- journal article
- research article
- Published by Wiley in Journal of Comparative Neurology
- Vol. 292 (1), 1-53
- https://doi.org/10.1002/cne.902920102
Abstract
Acetylcholine (ACh) plays a major role in central autonomic regulation, including the control of arterial blood pressure (AP). Previously unknown neuroanatomic substrates of cholinergic - autonomic control were mapped in this study. Cholinergic perikarya and bouton-like varicosities were localized by an immunocytochemical method empolying a monoclonal antiserum against choline acetyltransferase (ChAT), the enzyme synthesizing ACh. In the forebrain, bouton-like varicosities and/or perikarya were detected in the septum, bed nucleus of the stria terminalis, amygdala (in particular, autonomic projection areas AP1 and AP2 bordering the central subnucleus, hypothalamus rostrolateral/innominata transitional area, perifornical, dorsal, incertal, caudolateral, posterior [PHN], subparafascicular, supramammillary and mammillary nuclei. Few or no punctate varicosities were labeled in the paraventricular (PVN) or supraoptic (SON) hypothalamic nuclei. In the mid and hindbrain, immunoreactive cells and processes were present in the nucleus of Edinger-Westphal, periaqueductal gray, parabrachial complex (PBC), a periceruleal zone avoiding the locus ceruleus (LC), pontine micturition field, pontomedullary raphe, paramedian reticular formation and periventricular gray, A5 area, lateral tegmental field, nucleus tractus solitarii (NTS), nucleus commissuralis, nucleus reticularis rostroventrolateralis (RVL), and the ventral medullary surface (VMS). In the PBC, immunoreactive varicosities identified areas previously unexplored for cholinergic autonomic responsivity superior, internal, dorsal, and central division of the lateral subnucleus, nucleus of Koelliker-Fuse and the medial subnucleus. In the NTS, previously undescribed ChAT-immunolabeled cells and processes were concentrated at intermediate and subpostermal levels and distributed viscerotopically in areas receiving primary cardiopulmonary afferents. In the nucleus RVL, cholinergic perikarya were in proximity to the VMS and medial to adrenergic cell bodies of the C1 area. Punctate varicosities of unknown origin and dendrites extending ventrally from the nucleus ambiguus overlapped the C1 area and immediate surround of RVL. In conclusion: 1) Cholinergic perikarya and putative terminal fields, overlap structures that are rich in cholinoreceptors and express autonomic, neuroendocrine, or behavioral responsivity to central cholinergic stimulation (PHN, NTS, RVL). The role of ACh in most immunolabeled areas, however, has yet to be determined. Overall, these data support the concept that cholinergic agents act at multiple sites in the CNS and with topographic specificity. (2) The absence of immunoreactive elements in the LC, PVN, and SON was unexpected and suggests that cholinergic processing attributed to these nuclei is mediated polysynaptically or by synapses on processes extending into adjacent cholinoreceptor fields. (3) Putative cholinergic terminals overlapping sites that relay primary (NTS) or higher-order visceral afferents suggest anatomical substrates for cholinergic regulation of autonomic reflexes. (4) ChAT-immunoreactive terminals in areas where cells project to the IML support the view that central cholinergic stimulation provoking sympathoexcitation may be mediated by bulbospinal neurons. A rich plexus of varicose fibers overlapping the C7 area of RVL, which provides the excitatory drive for tonic sympathetic discharge, may form the anatomical basis for the increases in sympathetic nerve activity provoked by systemic or central administration of cholinergic agents.Keywords
This publication has 122 references indexed in Scilit:
- Changes in regional brain synaptosomal high affinity choline uptake during the development of hypertension in spontaneously hypertensive ratsNeurochemical Research, 1987
- The distribution of choline acetyltransferase in the rat amygdaloid complex and adjacent cortical areas, as determined by quantitative micro‐assay and immunohistochemistryJournal of Comparative Neurology, 1986
- Evidence for the coexistence of acetylcholine and enkephalin in the sympathetic preganglionic neurons of ratsBrain Research, 1985
- The architecture and some of the interconnections of the rat's amygdala and lateral periallocortexJournal of Comparative Neurology, 1984
- Atlas of catecholamine perikarya, varicosities and pathways in the brainstem of the catJournal of Comparative Neurology, 1983
- Innervation of the thymus gland by brain stem and spinal cord in mouse and ratJournal of Anatomy, 1981
- Pharmacological analysis of a cholinergic receptor mediated regulation of brain norepinephrine neuronsJournal of Neural Transmission, 1980
- Afferent projections to the dorsal and ventral respiratory nuclei in the medulla oblongata of the cat studied by the horseradish peroxidase techniqueBrain Research, 1980
- A description of the amygdaloid complex in the rat and cat with observations on intra‐amygdaloid axonal connectionsJournal of Comparative Neurology, 1978
- Topographic atlas of catecholamine and acetylcholinesterase‐containing neurons in the rat brain. II. Hindbrain (mesencephalon, rhombencephalon)Journal of Comparative Neurology, 1974