Disk Electrophoresis of Plasmodium berghei

Abstract

A method of analytic separation of soluble proteins in aqueous extracts of P. berghei is described. Extracts are prepared by sonication of supsensions of whole washed parasites obtained by saponin lysis of infected rat red cells. Separation of the extracts into reproducible electro-phoretic patterns is carried out by zone electrophoresis on polyacryla-mide gel. Extracts prepared in this manner contain a heterogenous mixture of soluble protein constituents of both host and parasite origin. A minimum of 15 parasite proteins and an additional minimum of six proteins of host origin have been identified.