A Method for Making Aceto-Carmine Squashes Permanent Without Removal of the Cover Slip

Abstract

Two modifications of Bridges' alcohol vapor method for making smear and squash preparations permanent are described. The variation of more general significance permits euparal to be applied without removal of the cover slip. Thus, possibility of loss, distortion or overlapping of desirable material is eliminated from this phase of preparation. The second modification is a reduction in duration of the alcohol vapor treatment. Although the latter alteration in schedule has no particular value in connection with material of many genera, it allows the vapor method to be applied to tissues of Nicotiana and may be useful in dealing with material of some other genera.