Studies on the deoxyribonucleases of bacteriophage-infected Escherichia coli
- 1 December 1962
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 85 (3), 600-606
- https://doi.org/10.1042/bj0850600
Abstract
The high deoxyribonuclease (DNA-ase) activity in extracts from E. coli infected with phages T2, T5 or T6 is due to synthesis of one or more enzymes after infection. The increase was greatest when (DNA-ase) activity was measured at about pH 9 with heat-denatured deoxyribonucleic acid (DNA) as substrate. The (DNA-ase) activity after infection with T2 was markedly more sensitive to inhibition by 30 [mu]M-p-chloromercuribenzoate than that before or that after infection with T5. The increase of (DNA-ase) activity did not start until most of the bacterial DNA had been degraded to acid-soluble material in T5-infected cultures. The increase occurred at about the same time as the increase of the activity responsible for the phosphorylation of thymidylate.Keywords
This publication has 23 references indexed in Scilit:
- Synthesis of protein in a purine-requiring Escherichia coli infected with bacteriophage T2Biochemical Journal, 1960
- Nucleic acid metabolism in Escherichia coli infected with phage T5Virology, 1959
- Deoxyribonucleic acid breakdown and resynthesis in T5 bacteriophage infectionVirology, 1958
- Lysis inhibition with a mutant of bacteriophage T5Virology, 1958
- A new method for the isolation of ribonucleic acids from mammalian tissuesBiochemical Journal, 1956
- THE LARGE SCALE PREPARATION OF SODIUM DESOXYRIBONUCLEATE FROM RIPE SALMON TESTESJournal of Biological Chemistry, 1953
- ORIGIN AND FATE OF BACTERIOPHAGE MATERIALCold Spring Harbor Symposia on Quantitative Biology, 1953
- The increase in desoxyribonuclease of virus-infected E. coliArchives of Biochemistry and Biophysics, 1952
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951
- A PRESS FOR DISRUPTING BACTERIA AND OTHER MICRO-ORGANISMS1951