HISTOCHEMICAL DEMONSTRATION OF N-ACETYL-β-GLUCOSAMINIDASE EMPLOYING NAPHTHOL AS-BI N-ACETYL-β-GLUCOSAMINIDE AS SUBSTRATE
- 1 May 1965
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 13 (5), 355-360
- https://doi.org/10.1177/13.5.355
Abstract
The N-acetyl-β-glucosaminide of naphthol AS-BI (7-bromo-3-hydroxy-2-naphth-o-anisidide) was obtained by reacting the anisidide with acetochloroglucosamine in aqueous alkaline acetone. After removal of O-acetyl groups with methanolic ammonia and recrystallization from aqueous methanol, the compound may be used as a substrate for N-acetyl-β-glucosaminidase.Keywords
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- Tissue fractionation studies. 15. Intracellular distribution and properties of β-N-acetylglucos-aminidase and β-galactosidase in rat liverBiochemical Journal, 1960
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- Studies on glucosaminidase. N-Acetyl-β-glucosaminidase in rat kidneyBiochemical Journal, 1957