Isolation of noninfectious particles containing Rous sarcoma virus RNA from the medium of Rous sarcoma virus-transformed nonproducer cells.

Abstract

Chick embryo cells infected and transformed by the Bryan high-titer strain of RSV [Rous sarcoma virus] produce noninfectious particles with physical and chemical characteristics similar to those of RSV + RAV [Rous associated virus]. The single-stranded RNA contained in the particles is indistinguishable in sedimentation velocity and base composition from RSV + RAV RNA.