Mouse lysozyme M gene: isolation, characterization, and expression studies.
- 1 September 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (17), 6232-6236
- https://doi.org/10.1073/pnas.85.17.6232
Abstract
We have isolated and characterized both cDNA and genomic DNA of the mouse lysozyme M gene. Derivation of the amino acid sequence from the nucleotide sequences revealed six positions in the carboxyl terminus that differ from partial sequences previously published. The differential detection of specific mRNAs from the closely related lysozyme M and P genes has revealed different but overlapping tissue specificities of expression. The M gene is expressed weakly in myeloblasts, moderately in immature macrophages, and strongly in both mature macrophages and macrophage-rich tissues, while high levels of P transcripts are present only in small intestine. Sites of protein accumulation, rather than gene expression, have been identified by comparative quantitation of mRNA and enzyme levels.Keywords
This publication has 43 references indexed in Scilit:
- Murine macrophage cell lines can be ordered in a linear differentiation sequenceDifferentiation, 1986
- Transformation of mononuclear phagocytes in vivo and malignant histiocytosis caused by a novel murine spleen focus-forming virusNature, 1985
- Organization and Expression of Eucaryotic Split Genes Coding for ProteinsAnnual Review of Biochemistry, 1981
- 3′ Non-coding region sequences in eukaryotic messenger RNANature, 1976
- Tissue distribution of lysozyme in man.Journal of Histochemistry & Cytochemistry, 1975
- Lactose biosynthesisReviews of physiology, biochemistry and pharmacology, 1975
- LOCALIZATION OF LYSOZYME IN NORMAL RAT TISSUES BY AN IMMUNOPEROXIDASE METHODJournal of Histochemistry & Cytochemistry, 1974
- Differentiation of a cell line of myeloid leukemiaJournal of Cellular Physiology, 1969
- SERUM AND URINARY LYSOZYME (MURAMIDASE) IN MONOCYTIC AND MONOMYELOCYTIC LEUKEMIAThe Journal of Experimental Medicine, 1966
- Deletion of thymidine kinase activity from L cells resistant to bromodeoxyuridineExperimental Cell Research, 1963