Maturation of mouse fetal germ cells in vitro
Open Access
- 1 August 2002
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 418 (6897), 497
- https://doi.org/10.1038/418497a
Abstract
Nuclear reprogramming is essential during gametogenesis for the production of totipotent zygotes. Here we show that premeiotic female germ cells derived from mouse fetuses as early as 12.5 days post coitum are able to complete meiosis and genomic imprinting in vitro and that these matured oocytes are highly competent in supporting development to full term after nuclear transfer and in vitro fertilization. To our knowledge, this is the first time that complete oogenesis has been successfully accomplished in vitro.Keywords
This publication has 10 references indexed in Scilit:
- Methylation Dynamics of Imprinted Genes in Mouse Germ CellsGenomics, 2002
- Maternal Primary Imprinting Is Established at a Specific Time for Each Gene throughout Oocyte GrowthPublished by Elsevier ,2002
- Dnmt3L and the Establishment of Maternal Genomic ImprintsScience, 2001
- Epigenetic Modifications Necessary for Normal Development Are Established During Oocyte Growth in Mice1Biology of Reproduction, 2000
- Imprinted expression of the Igf2r gene depends on an intronic CpG islandNature, 1997
- ‘Green mice’ as a source of ubiquitous green cellsFEBS Letters, 1997
- Ovary and ovulation: In-vitro maturation, fertilization and embryo development of immature oocytes from early preantral follicles from prepuberal mice in a simplified culture systemHuman Reproduction, 1996
- Epigenetic modifications during oocyte growth correlates with extended parthenogenetic development in the mouseNature Genetics, 1996
- Development in vitro of mouse oocytes from primordial folliclesBiology of Reproduction, 1996
- Expression of glucose‐phosphate isomerase in relation to growth of the mouse oocyte in vivo and in vitroGamete Research, 1985