A comparison of sister chromatid exchange frequencies in peripheral lymphocytes and bone marrow cells of Fischer 344 and Sprague-Dawley rats
- 1 January 1984
- journal article
- research article
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 38 (3), 216-220
- https://doi.org/10.1159/000132063
Abstract
We have investigated spontaneous sister chromatid exchange (SCE) frequencies in peripheral lymphocytes and bone marrow cells explanted from two strains of the laboratory rat, Fischer 344 and Sprague-Dawley. A small, but significant difference was noted for both cell types, with the Fischer 344 rat being consistently higher. Other cell parameters, such as the mitotic index and the replicative index, were similar in the two strains. SCE levels in cultured peripheral lymphocytes after intraperitoneal administration of the alkylating drug cyclophosphamide (10 mg/kg) were similar for the two strains. Fischer 344 rats are known to have approximately a 10-fold higher incidence of spontaneous leukemia than do Sprague-Dawley rats. Since SCE frequency is a sensitive measure of DNA damage, our observations suggest that high leukemia incidence in the Fischer 344 rat may be related to a higher level of spontaneous DNA damage.This publication has 17 references indexed in Scilit:
- Sister-chromatid exchanges: A report of the GENE-TOX programMutation Research/Reviews in Genetic Toxicology, 1981
- Carcinogenic action of low‐dose cyclophosphamide given orally to sprague‐dawley rats in a lifetime experimentInternational Journal of Cancer, 1979
- Spontaneous and mitomycin-C induced sister-chromatid exchangesMutation Research, 1979
- In vivo sister-chromatid exchange: A sensitive measure of DNA damageMutation Research, 1979
- Sister chromatid exchange in murine alveolar macrophages, regenerating liver and bone marrow cells ? a simultaneous multicellular in vivo assayChromosoma, 1979
- Sister chromatid exchanges in human leukocyte chromosomes: Spontaneous and induced frequencies in early- and late-proliferating cells in vitroHuman Genetics, 1978
- Direct evidence for the role of incorporated BUdR in the induction of sister chromatid exchangesExperimental Cell Research, 1978
- An increased frequency of chromosomal changes and SCE's in cultured blood lymphocytes of 12 subjects vaccinated against smallpoxHuman Genetics, 1978
- A simplified technique for in vivo analysis of sister-chromatid exchanges using 5-bromodeoxyuridine tabletsCytogenetic and Genome Research, 1977
- In vivo BrdU-33258 Hoechst analysis of DNA replication kinetics and sister chromatid exchange formation in mouse somatic and meiotic cellsChromosoma, 1976