Abstract
An extremely simple yet highly specific method was evolved (by a committee of collaborators) for the detn. of carotene in dried grass meal. The meal (1 to 2g.) is heated with light petroleum of b.p. 80.100[degree]C in a longnecked flask on a steam bath for 1 hr. Non-carotene pigments are removed on a column of bone meal. A collaborative comparison between the method and an older one using phase partition showed that 31% of the pigment estimated in the older method was not true carotene.