CO2 laser urethroplasty in the rabbit: A preclinical model

Abstract

Our previous work has shown that the CO₂ laser can be successfully used in urethral reconstruction in a rat model. This new experiment investigates the use of the CO₂ laser to perform a patch graft urethroplasty in the rabbit, as a preclinical model to its use in the repair of hypospadias in humans. Using sterile technique, a patch graft of preputial skin was welded in the repair of a standardized urethral defect in 10 rabbits. In another cohort, the same urethral defect was repaired using standard microsuture technique. In a control group the patch graft was placed with microsuture in a nonwatertight fashion. All animals were followed for 3 weeks. Histologic and radiologic analyses were done in a blinded fashion. Our study showed that CO₂ laser repair, when compared to microsuture in urethral reconstruction, required 40% less operative time and produced better graft healing and less intraluminal scarring.