CHARACTERIZATION OF 2 DIFFERENT AGGLUTINATORS IN LATEX FIXATION TEST OCCURRING IN NORMAL HUMAN SERA

Abstract

Using a sensitive modification of the latex fixation test it is possible to detect a small agglutinating effect in about 60% of normal human sera, after these have been heated for 30 minutes at 56[degree]. This was shown to be caused by an IgM globulin with the properties of a rheumatoid factor. The factor is able to react with human IgG globulin and may represent an antibody to the IgG part of circulating antigen-antibody complexes. The heat treatment probably inactivates an inhibitor of the latex fixation reaction. In addition all normal human sera give an agglutination reaction with IgG coated latex at incubation temperatures of 37[degree] or lower. It was shown that these reactions are caused by a thermolabile, non-reducible component with a sedimentation constant of about 10. This component is probably identical with the complement component C1q, The agglutinating activity was found in the [alpha]2-B1 region after electrophoresis of untreated serum but in the slow y region after treatment of the serum with ethylenedi-nitrilo tetra-acetic acid. This kind of agglutination may cause false positive reactions in latex tests which are carried out at 37[degree] or less.