Growth of a Candidate Arbovirus (Tsuruse) in Aedes aegypti Mosquitoes Following Intrathoracic Inoculation

Abstract

Tsuruse virus, a candidate arbovirus, isolated in mice from bird (Blue Magpie) blood in Japan, grew unequivocally in A. acgypti after intrathoracic inoculation, though in only a rare mosquito after oral feeding. The estimated size of the virus was 50-100 mμ by filtration, and it was inactivated by lipid solvents, grew in the presence of iododeoxyuridine, produced plaques in cultures of primary chicken embryonic cells under agar and was lethal to suckling and weanling mice and weanling hamsters inoculated intracranially and to chicken embryos after yolk sac inoculation. No antigenic relationships were found between Tsuruse virus and 112 arboviruses.