Comparison of methods for the detection of biofilm production in coagulase-negative staphylococci
Open Access
- 14 October 2010
- journal article
- Published by Springer Nature in BMC Research Notes
- Vol. 3 (1), 260
- https://doi.org/10.1186/1756-0500-3-260
Abstract
The ability of biofilm formation seems to play an essential role in the virulence of coagulase-negative staphylococci (CNS). The most clearly characterized component of staphylococcal biofilms is the polysaccharide intercellular adhesin (PIA) encoded by the icaADBC operon. Biofilm production was studied in 80 coagulase-negative staphylococci (CNS) strains isolated from clinical specimens of newborns with infection hospitalized at the Neonatal Unit of the University Hospital, Faculty of Medicine of Botucatu, and in 20 isolates obtained from the nares of healthy individuals without signs of infection. The objective was to compare three phenotypic methods with the detection of the icaA, icaD and icaC genes by PCR.Keywords
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