The Extraction and Colorimetric Estimation of Indole-3-Acetic Acid and Its Esters in Developing Corn Kernels

Abstract
A colorimetric method for the separate determinations of IAA and its esters as they occur in developing corn kernels is presented. While some IAA was present at all stages except in the mature kernels, it reached a peak in fresh corn during the intermediate milk stage. The tomato fruit-setting factor, ethylindole-3-acetate was found only in kernels which had matured to[long dash]or beyond[long dash]the intermediate milk stage. The highest concentration of the ester occurred at the same stage of development which characterized the peak in concentration of IAA.