Antioxidant Protection by American Ginseng in Pancreatic ß-Cells
Open Access
- 1 January 2008
- journal article
- research article
- Published by World Scientific Pub Co Pte Ltd in The American Journal of Chinese Medicine
- Vol. 36 (05), 981-988
- https://doi.org/10.1142/s0192415x08006399
Abstract
Hyperglycemia in diabetic conditions may cause oxidative stress in pancreatic ß-cells, leading to their dysfunction and insulin resistance within peripheral tissues. Previous studies suggest that American ginseng berry extract may have hypoglycemic effects, as well as offer antioxidant protection. We examined effects of American ginseng berry extract and ginsenoside Re in a pancreatic ß-cell line, MIN-6, to determine if these two properties are related. Cells were exposed to oxidative stress via hydrogen peroxide incubation and oxidative stress was measured by oxidation of 2′,7′-dichlorofluorescin diacetate. These cells showed a concentration-related response to hydrogen peroxide at 100–500 μM. In acute conditions where cells were treated with the extract for 10 min, we observed reduced oxidant injury suggesting direct scavenging effects. Chronic incubation of cells with the extract for 48 hours also demonstrated attenuation of oxidative stress. At high concentrations, Re showed a mild antioxidant effect in MIN-6 cells. Our insulin release observations also showed that the extract may help to increase insulin secretions from the cells. Our data suggest that the observed ability of ginseng to reduce blood glucose levels may be linked to its antioxidant effects on pancreatic ß-cells.Keywords
This publication has 25 references indexed in Scilit:
- Commonly Used Antioxidant Botanicals: Active Constituents and Their Potential Role in Cardiovascular IllnessThe American Journal of Chinese Medicine, 2007
- Antioxidant Effect of Wen-Pi-Tang and Its Component Crude Drugs on Oxidative StressThe American Journal of Chinese Medicine, 2007
- Protective Effects ofGinkgo biloba,Panax ginseng, andSchizandra chinensisExtract on Liver Injury in RatsThe American Journal of Chinese Medicine, 2007
- Application of Proteomics in Chinese Medicine ResearchThe American Journal of Chinese Medicine, 2007
- Saponins Composition in American Ginseng Leaf and Berry Assayed by High-Performance Liquid ChromatographyJournal of Agricultural and Food Chemistry, 2006
- Anti-hyperglycemic Activity of Natural and FermentedCordyceps sinensisin Rats with Diabetes Induced by Nicotinamide and StreptozotocinThe American Journal of Chinese Medicine, 2006
- Hydrogen Peroxide Alters Mitochondrial Activation and Insulin Secretion in Pancreatic Beta CellsJournal of Biological Chemistry, 1999
- Miniglucagon (Glucagon 19–29), a Potent and Efficient Inhibitor of Secretagogue-induced Insulin Release through a Ca2+PathwayPublished by Elsevier ,1999
- Panax quinquefolium saponins protects low density lipoproteins from oxidationLife Sciences, 1998
- Ginseng Therapy in Non-Insulin-Dependent Diabetic Patients: Effects on psychophysical performance, glucose homeostasis, serum lipids, serum aminoterminalpropeptide concentration, and body weightDiabetes Care, 1995