Three‐dimensional structure of renal Na,K‐ATPase determined by electron microscopy of membrane crystals
- 22 July 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 187 (1), 182-186
- https://doi.org/10.1016/0014-5793(85)81238-2
Abstract
The three-dimensional structure of Na,K-ATPase was determined by electron microscopy and image processing. Tilt series of negatively stained membrane crystals were recorded. The projections were analyzed by Fourier methods and the data combined to a 3-D model. The unit cell contains two rod-shaped stain-deficient regions interpreted as αβ-protomers of Na,K-ATPase. The rods are related by dyad axes oriented perpendicular to the membrane. Outside the lipid bilayer the rods contact different protein units on the two sides of the membraneKeywords
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